簡(jiǎn)介：艾睿生物提供KYSE30細(xì)胞(1411D)實(shí)時(shí)價(jià)格和產(chǎn)品詳細(xì)說(shuō)明,敬請(qǐng)來(lái)電咨詢!詳細(xì)介紹KYSE30細(xì)胞(1411D)。
詳細(xì)說(shuō)明：
KYSE30細(xì)胞(1411D)——含 量：99 產(chǎn)品規(guī)格：100000-1000000cells/ml 產(chǎn)品包裝：液體瓶裝 品 牌：ATCC細(xì)胞庫(kù)、ScienCell實(shí)驗(yàn)室等細(xì)胞提供單位 產(chǎn) 地：美國(guó)、經(jīng)銷
細(xì)胞規(guī)格：100000-1000000cells/ml
儲(chǔ)存：液氮(凍存)或復(fù)蘇培養(yǎng)
運(yùn)輸：干冰 用途：細(xì)胞領(lǐng)域科學(xué)研究
KYSE30細(xì)胞(1411D)于原代凍存或復(fù)蘇培養(yǎng)(這個(gè)細(xì)胞提供的周期會(huì)長(zhǎng)點(diǎn)，少則半個(gè)月，多則一兩個(gè)月)。每管含有細(xì)胞數(shù)>5×100000cells/ml，此細(xì)胞通過(guò)**熒光染色驗(yàn)證，經(jīng)測(cè)試不含有HIV-1、HBV、HCV、支原體、**、酵母和**。細(xì)胞可以達(dá)到10倍增。 公司長(zhǎng)期提供細(xì)胞產(chǎn)品，很多細(xì)胞科研單位都特意訂購(gòu)我公司X細(xì)胞，經(jīng)常購(gòu)買我們公司細(xì)胞產(chǎn)品的單位有：天津醫(yī)科大學(xué)、寧夏醫(yī)科大學(xué)、**軍醫(yī)大學(xué)、徐州醫(yī)學(xué)院、哈爾濱醫(yī)科大學(xué)、廣州中醫(yī)藥大學(xué)、第三軍醫(yī)大學(xué)、上海交大醫(yī)學(xué)院、蘭州大學(xué)、貴州省遵義市遵義醫(yī)學(xué)院、湖北武漢同濟(jì)醫(yī)院神經(jīng)外科、江蘇省人民醫(yī)院、北京大學(xué)人民醫(yī)院等單位的重點(diǎn)實(shí)驗(yàn)室。
細(xì)胞凍存和復(fù)蘇實(shí)驗(yàn)原理:細(xì)胞凍存及復(fù)蘇的基本原則是慢凍快融，實(shí)驗(yàn)證明這樣可以限度的保存細(xì)胞活力。目前細(xì)胞凍存多采用甘油或二甲基亞砜作保護(hù)劑，這兩種物質(zhì)能提高細(xì)胞膜對(duì)水的通透性，加上緩慢冷凍可使細(xì)胞內(nèi)的水分滲出細(xì)胞外，減少細(xì)胞內(nèi)冰晶的形成，從而減少由于冰晶形成造成的細(xì)胞損傷。復(fù)蘇細(xì)胞應(yīng)采用快速融化的方法，這樣可以保證細(xì)胞外結(jié)晶在很短的時(shí)間內(nèi)即融化，避免由于緩慢融化使水分滲入細(xì)胞內(nèi)形成胞內(nèi)再結(jié)晶對(duì)細(xì)胞造成損傷。
細(xì)胞凍存和復(fù)蘇實(shí)驗(yàn)注意事項(xiàng):
1. 從增殖期到形成致密的單層細(xì)胞以前的培養(yǎng)細(xì)胞都可以用于凍存，但好為對(duì)數(shù)生長(zhǎng)期細(xì)胞。在凍存前**好換一次培養(yǎng)液。
2. 將凍存管放入液氮容器或從中取出時(shí)，要做好防護(hù)工作，以免**。
3. 凍存和復(fù)蘇好用新配制的培養(yǎng)液。
人肺動(dòng)脈平滑肌細(xì)胞凍存和復(fù)蘇的原則：慢凍快融
當(dāng)細(xì)胞冷到零度以下，可以產(chǎn)生以下變化：細(xì)胞器脫水，細(xì)胞中可溶性物質(zhì)濃度升高，并在細(xì)胞內(nèi)形成冰晶。
如果緩慢冷凍，可使細(xì)胞逐步脫水，細(xì)胞內(nèi)不致產(chǎn)生大的冰晶;相反，結(jié)晶就大，大結(jié)晶會(huì)造成細(xì)胞膜、綱胞器的損傷和破裂。復(fù)蘇過(guò)程應(yīng)快融，目的是防止小冰晶形成大冰晶，即冰晶的重結(jié)晶。
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